Label-free Sensing of Main Protease Activity of SARS-CoV-2 with an Aerolysin NanoporeArticle Published on 2022-11-022022-11-15 Journal: Chemistry, an Asian journal [Category] SARS, 치료기술, [키워드] activity acute respiratory syndrome amplification Biomarker cleavage cleaved Concentration conserved coronavirus Critical diagnostic enzyme events exhibited expand M pro MPro Nanopore peptide produced protease Replication SARS-CoV-2 sensitivity sensors. translocated viruses [DOI] 10.1002/asia.202200747 PMC 바로가기
Reactive Centre Loop Mutagenesis of SerpinB3 to Target TMPRSS2 and Furin: Inhibition of SARS-CoV-2 Cell Entry and ReplicationArticle Published on 2022-10-192022-11-15 Journal: International Journal of Molecular Sciences [Category] COVID19(2023년), SARS, 변종, 치료제, [키워드] Alpha Altered Anti-viral Anti-viral therapy Antiviral assays biological molecules cathepsin L Cell cell entry cellular proteases cleaved designer serpin effective enzyme expressing facilitate furin furin. generate host cell protease implicated in vitro infection with SARS-CoV-2 inhibit inhibition inhibitor inhibitors inhibitory inhibitory activity loop membrane fusion Mutagenesis P681R predicted produced protease Protein purified rate constant reactive Recombinant protein S protein cleavage S1/S2 cleavage site SARS-CoV-2 SARS-CoV-2 replication SARS-CoV-2 viral replication SARS-CoV-2 virus serine 2 serine protease inhibitor SerpinB3 shown specificity suppress target the Spike the spike protein TMPRSS2 transmembrane variant Vero E6 cell [DOI] 10.3390/ijms232012522 PMC 바로가기
Serum angiotensin-converting enzyme 2 as a potential biomarker for SARS-CoV-2 infection and vaccine efficacyArticle Published on 2022-10-122022-11-15 Journal: Frontiers in Immunology [Category] COVID19(2023년), SARS, 바이오마커, [키워드] ACE2 angiotensin-converting enzyme 2 Biomarker change changes in circulating cleaved COVID-19 death declined demonstrated Disease progression dose Efficacy elevated expressing full-length highlight host cell receptor human ACE2 humans immunization increase in K18-hACE2 mice membrane mice MVA-S vaccine prevented Proteolytic processing receptor SARS-CoV-2 SARS-COV-2 infection serum serum sample serum. subsequent susceptible the SARS-CoV-2 transgenic vaccination Vaccine vaccine candidate virus virus entry [DOI] 10.3389/fimmu.2022.1001951 PMC 바로가기
The Spike-Stabilizing D614G Mutation Interacts with S1/S2 Cleavage Site Mutations To Promote the Infectious Potential of SARS-CoV-2 VariantsArticle Published on 2022-10-122022-11-16 Journal: Journal of Virology [Category] COVID19(2023년), SARS, 변종, [키워드] acute respiratory syndrome addition association carried Cell cleavage cleavage site cleaved coronavirus D614G D614G mutation Delta dependent on epistasis epistatic interaction evaluate expressing expression increasingly Mutation omicron pandemic pathogenic Potential Protein proteolytic proteolytic cleavage pseudovirions pseudovirus pseudoviruses remained resulting S1 subunit S1/S2 S1/S2 cleavage S1/S2 cleavage site SARS-CoV-2 SARS-CoV-2 variant Site spike spike mutation spikes Spread stabilizing effect successive wave Transmissibility variant variants variants of concern variants. Viral particles viral spike viral variant virions wild-type Wuhan [DOI] 10.1128/jvi.01301-22 PMC 바로가기
Variation of Proteolytic Cleavage Sites towards the N-Terminal End of the S2 Subunit of the Novel SARS-CoV-2 Omicron Sublineage BA.2.12.1Article Published on 2022-09-082022-11-16 Journal: Molecules [Category] SARS, 변종, [키워드] accompanied Activation amino acid substitution BA.2.12.1 binding cathepsin G Cell cleavage site cleaved complex COVID-19 digested End furin immune response Infection MHC modify neutrophil Neutrophil elastase Neutrophils novel omicron Omicron variant Pathogens peptide polybasic Prevalence priming protease Protein proteinase proteinase 3 S1/S2 S2 antigen S2 subunit SARS-CoV-2 SARS-CoV-2 variant secrete sequence serine protease serine proteases serine proteases. Site Support T cell activation the major histocompatibility the S protein the Spike TMPRSS2 viral entry [DOI] 10.3390/molecules27185817 PMC 바로가기
Structural investigations of proteins encoded by SARS-CoV-2Article Published on 2022-09-012022-11-16 Journal: FEBS Open Bio [Category] SARS, 변종, 유전자 메커니즘, [키워드] antibodies articles bases categories cellular cleaved commenced coronavirus COVID COVID-19 pandemic cysteine proteases described ENCODE Evolution example expressed Final Genome include knowledge MN908947.3 molecular mechanism other coronaviruses pandemic polyprotein positive-sense Protein protein structure purified reported Research ribonuclease RNA genome RNAs SARS-CoV-2 selected sequenced single-stranded small molecule drugs Spike protein spike protein. structural property the disease the spike protein translation Vaccines variant viral transcript were expressed [DOI] 10.1002/2211-5463.13465 PMC 바로가기
Metalloproteinase-Dependent and TMPRSS2-Independent Cell Surface Entry Pathway of SARS-CoV-2 Requires the Furin Cleavage Site and the S2 Domain of Spike ProteinArticle Published on 2022-08-302022-11-16 Journal: mBio [Category] COVID19(2023년), SARS, 변종, 유전자 메커니즘, [키워드] ACE2 ADAM10 addition bind Cell cell death cell interaction cleavage cleaved complex condition contributed coronavirus SARS-CoV COVID-19 develop domain effective element endosomal pathway entry pathway evade furin furin cleavage motif highly pathogenic Host host protease human coronaviruses identify Immunity independent Infection infection mechanism inhibitor involved membrane membrane fusion metalloproteinase organ damage Pathogenesis pathogenesis of COVID-19 pathway Prevent priming provide required S1/S2 boundary S2 domain SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 spike protein selective sequence siRNAs Site spike Spread surface surface receptor syncytium formation the spike protein therapeutic agent therapeutic target Therapies TMPRSS2 unique vaccination variants virus entry Virus entry. virus variant [DOI] 10.1128/mbio.00519-22 PMC 바로가기
Protocol to isolate and assess spike protein cleavage in SARS-CoV-2 variants obtained from clinical COVID-19 samplesArticle Published on 2022-06-132022-10-05 Journal: STAR protocols [Category] COVID19(2023년), SARS, 변종, 진단, 치료기술, [키워드] cell entry cellular proteases cleavage cleaved Complete COVID-19 sample ENhance Evolutionary biology facilitate in viral include Isolation Microbiology Nasopharyngeal swab patients with COVID-19 protocol SARS-CoV-2 SARS-CoV-2 spike protein SARS-CoV-2 variant SARS-CoV-2-infected cells spike mutation Spike protein supernatant [DOI] 10.1016/j.xpro.2022.101502 [Article Type] Article
Structure and activity of human TMPRSS2 protease implicated in SARS-CoV-2 activation Research Published on 2022-06-082022-10-05 Journal: Nature chemical biology [Category] COVID19(2023년), SARS, 치료제, [키워드] Activation airways binding pocket canonical cleaved effort explain feature half-maximal inhibitory concentration host cell identify implicated Infection inhibit inhibitor mechanism membrane fusion nonspecific pathogenesis of SARS-CoV-2 protease Protease inhibitor Protein proteolytic activity provide S1/S2 cleavage site SARS-CoV-2 SARS-CoV-2 S protein serine 2 specificity spike Structure the SARS-CoV-2 TMPRSS2 TMPRSS2 protease viral entry X-ray [DOI] 10.1038/s41589-022-01059-7 [Article Type] Research
Pyroptosis and Its Role in SARS-CoV-2 InfectionPyroptosis와 SARS-CoV-2 감염에서의 역할Review Published on 2022-05-232022-09-12 Journal: Cells [Category] COVID19(2023년), MERS, SARS, 진단, [키워드] activated Caspases cleaved contribute COVID-19 pathology described disease downstream event excessive inflammation gasdermins GSDMD Health humans Inflammasome Inflammatory cell death inhibitor interleukin-1β molecular NLRP3 NLRP3 inflammasome pathway pyroptosis pyroptosis. role SARS-CoV-2 [DOI] 10.3390/cells11101717 PMC 바로가기 [Article Type] Review